pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-M566T
(Plasmid #218832)

• Purpose: Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant M566T for BioID experiments.
• Depositing Lab: Travis Stracker
• Publication: Identification of de novo mutations in TLK1 and MDM1 in a patient with a neurodevelopmental disorder and immunodeficiency (unpublished)

Backbone

• Vector backbone: pcDNA 5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5037
• Total vector size (bp): 8323
• Modifications to backbone: FLAG-BirA* added before MCS
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TLK1 M566T
• Species: H. sapiens (human)
• Insert Size (bp): 2157
• Mutation: M566T
• GenBank ID: NM_001136554.2
• Entrez Gene: TLK1 (a.k.a. PKU-beta)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG-BirA* (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was constructed in part with cDNAs for TLK1 and TLK2 from the Human ORFeome Version 1.1 library

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry