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Addgene

mP58.FL1-pCDNA3
(Plasmid #21883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P58
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1841
  • Entrez Gene
    Dnajc3 (a.k.a. AA408985, AU067833, Dnaj, Dnajc, Dnajc3a, Dnajc3b, P, Prkri, mp5, mp58, p58, p58IPK)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mP58.FL1-pCDNA3 was a gift from David Ron (Addgene plasmid # 21883 ; http://n2t.net/addgene:21883 ; RRID:Addgene_21883)

  • For your References section:

    Cotranslocational degradation protects the stressed endoplasmic reticulum from protein overload. Oyadomari S, Yun C, Fisher EA, Kreglinger N, Kreibich G, Oyadomari M, Harding HP, Goodman AG, Harant H, Garrison JL, Taunton J, Katze MG, Ron D. Cell. 2006 Aug 25. 126(4):727-39. 10.1016/j.cell.2006.06.051 PubMed 16923392
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