pcDNA-iLACCO1
(Plasmid
#218821)
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PurposeMammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiLACCO1
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SpeciesOther
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-iLACCO1 was a gift from Robert Campbell (Addgene plasmid # 218821 ; http://n2t.net/addgene:218821 ; RRID:Addgene_218821) -
For your References section:
High-Performance Genetically Encoded Green Fluorescent Biosensors for Intracellular l-Lactate. Hario S, Le GNT, Sugimoto H, Takahashi-Yamashiro K, Nishinami S, Toda H, Li S, Marvin JS, Kuroda S, Drobizhev M, Terai T, Nasu Y, Campbell RE. ACS Cent Sci. 2024 Jan 31;10(2):402-416. doi: 10.1021/acscentsci.3c01250. eCollection 2024 Feb 28. 10.1021/acscentsci.3c01250 PubMed 38435524