IRE1b(K536A).9E10.pCDNA3
(Plasmid #21881)

• Depositing Lab: David Ron
• Publication: IRE1 plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IRE1b
• Species: M. musculus (mouse)
• Insert Size (bp): 2806
• Mutation: K536A
• Entrez Gene: Ern2 (a.k.a. Ern1, Ire1, Ire1b, ire1-beta, mIre1)
• Tag / Fusion Protein:
  • Myc (9E10) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results show an additional mutation in the coding region, causing L850P substitution in the amino acid sequence.

Mouse IRE1b, K536A, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, in pCDNA3. The K->A mutation introduces a second MSc1 site into the plasmid at position 2578.