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Addgene

pBa.GFP-AP1/beta1
(Plasmid #218802)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218802 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBeta-Actin-GFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP1 b1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    753
  • Entrez Gene
    AP1B1 (a.k.a. ADTB1, AP105A, BAM22, CLAPB2, KIDAR)
  • Promoter Chicken Beta actin
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBa.GFP-AP1/beta1 was a gift from Marvin Bentley (Addgene plasmid # 218802 ; http://n2t.net/addgene:218802 ; RRID:Addgene_218802)

  • For your References section:

    Polarized transport requires AP-1-mediated recruitment of KIF13A and KIF13B at the trans-Golgi. Montgomery AC, Mendoza CS, Garbouchian A, Quinones GB, Bentley M. Mol Biol Cell. 2024 Mar 6:mbcE23100401. doi: 10.1091/mbc.E23-10-0401. 10.1091/mbc.E23-10-0401 PubMed 38446634
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