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IRE1b.WT.9E10.pCDNA3
(Plasmid #21880)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21880 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRE1b
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2806
  • Entrez Gene
    Ern2 (a.k.a. Ern1, Ire1, Ire1b, ire1-beta, mIre1)
  • Tag / Fusion Protein
    • Myc (9E10) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse IRE1b, wild-type, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, in pCDNA3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IRE1b.WT.9E10.pCDNA3 was a gift from David Ron (Addgene plasmid # 21880 ; http://n2t.net/addgene:21880 ; RRID:Addgene_21880)
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