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kiCAP-AAV-BI-hTFR1
(Plasmid #218796)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218796 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    iCap-Kanamycin
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAV Rep-Cap BI-hTFR1
  • Species
    Synthetic

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note on the use of the BI-hTFR1 capsids:
BI-hTFR1 and the other capsids described in this study bind specifically to the human Transferrin receptor and do not detectably bind the Transferrin receptor (TfR1) from other species. Therefore, users of these plasmids are advised to use the capsids with a suitable humanized TFRC KI mouse model or in cells expressing human TfR1. No enhanced tropism should be expected in any other species, including macaques or other species of nonhuman primates. Please see the accompanying article or contact Dr. Deverman for more information regarding the use of these capsids.

Please visit https://doi.org/10.1101/2023.12.20.572615 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    kiCAP-AAV-BI-hTFR1 was a gift from Ben Deverman (Addgene plasmid # 218796 ; http://n2t.net/addgene:218796 ; RRID:Addgene_218796)

  • For your References section:

    An AAV capsid reprogrammed to bind human transferrin receptor mediates brain-wide gene delivery. Huang Q, Chan KY, Wu J, Botticello-Romero NR, Zheng Q, Lou S, Keyes C, Svanbergsson A, Johnston J, Mills A, Lin CY, Brauer PP, Clouse G, Pacouret S, Harvey JW, Beddow T, Hurley JK, Tobey IG, Powell M, Chen AT, Barry AJ, Eid FE, Chan YA, Deverman BE. Science. 2024 Jun 14;384(6701):1220-1227. doi: 10.1126/science.adm8386. Epub 2024 May 16. 10.1126/science.adm8386 PubMed 38753766
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