pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA
(Plasmid #218795)

• Purpose: AAV reporter genome plasmid
• Depositing Lab: Ben Deverman
• Publication: Huang et al Science. 2024 Jun 14;384(6701):1220-1227. doi: 10.1126/science.adm8386. Epub 2024 May 16.

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Backbone

• Vector backbone: AAV Genome
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CMV-CBA-hGBA1-cm-hGBA1-hGHpA
• Species: Synthetic

Cloning Information

• Cloning method: Gene Synthesis

Resource Information

• Supplemental Documents:
  • pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note on the use of the BI-hTFR1 capsids:
BI-hTFR1 and the other capsids described in this study bind specifically to the human Transferrin receptor and do not detectably bind the Transferrin receptor (TfR1) from other species. Therefore, users of these plasmids are advised to use the capsids with a suitable humanized TFRC KI mouse model or in cells expressing human TfR1. No enhanced tropism should be expected in any other species, including macaques or other species of nonhuman primates. Please see the accompanying article or contact Dr. Deverman for more information regarding the use of these capsids.

Please visit https://doi.org/10.1101/2023.12.20.572615 for bioRxiv preprint.