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pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr
(Plasmid #218767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEVOL-tac
  • Backbone size w/o insert (bp) 4091
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ATMY5
  • Growth instructions
    This plasmid can be grown in either ATMY1, ATMY5, or ATMY-C321 strains (Addgene #218770, 218773, or 218774)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EcTyrRS-pAAFRS-9
  • Species
    E. coli
  • Insert Size (bp)
    1272
  • Mutation
    Y37G, L71V, D182C, F183Y, L186C, D265R
  • Promoter tacI

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EcY-tRNA-TAG

After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr was a gift from Abhishek Chatterjee (Addgene plasmid # 218767 ; http://n2t.net/addgene:218767 ; RRID:Addgene_218767)

  • For your References section:

    Native Aminoacyl-tRNA Synthetase/tRNA Pair Drives Highly Efficient Noncanonical Amino Acid Incorporation in Escherichia coli. Ficaretta ED, Singha Roy SJ, Voss L, Chatterjee A. ACS Chem Biol. 2024 Jun 24. doi: 10.1021/acschembio.4c00221. 10.1021/acschembio.4c00221 PubMed 38913984
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