pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
(Plasmid #218766)

• Purpose: encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG
• Depositing Lab: Abhishek Chatterjee
• Publication: Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021/acschembio.4c00221.

Backbone

• Vector backbone: pEVOL-tac
• Backbone size w/o insert (bp): 4091
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ATMY5
• Growth instructions: This plasmid can be grown in either ATMY1, ATMY5, or ATMY-C321 strains (Addgene #218770, 218773, or 218774)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EcTyrRS-VSMA*
• Species: E. coli
• Insert Size (bp): 1272
• Mutation: Y37V, D167G, D182S, F183M, L186A, D265R
• Promoter: tacI

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pevol-tac-ectyrrs-vsma-lpp-trna_cuaectyr.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EcY-tRNA-TAG

After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.