pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
(Plasmid
#218766)
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Purposeencodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218766 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEVOL-tac
- Backbone size w/o insert (bp) 4091
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ATMY5
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Growth instructionsThis plasmid can be grown in either ATMY1, ATMY5, or ATMY-C321 strains (Addgene #218770, 218773, or 218774)
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEcTyrRS-VSMA*
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SpeciesE. coli
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Insert Size (bp)1272
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MutationY37V, D167G, D182S, F183M, L186A, D265R
- Promoter tacI
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EcY-tRNA-TAG
After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr was a gift from Abhishek Chatterjee (Addgene plasmid # 218766 ; http://n2t.net/addgene:218766 ; RRID:Addgene_218766) -
For your References section:
Native Aminoacyl-tRNA Synthetase/tRNA Pair Drives Highly Efficient Noncanonical Amino Acid Incorporation in Escherichia coli. Ficaretta ED, Singha Roy SJ, Voss L, Chatterjee A. ACS Chem Biol. 2024 Jun 24. doi: 10.1021/acschembio.4c00221. 10.1021/acschembio.4c00221 PubMed 38913984