pEvoltac EcW-TGA-h9
(Plasmid #218765)

• Purpose: encodes EcWRS-h9 and EcW-tRNA-TGA
• Depositing Lab: Abhishek Chatterjee
• Publication: Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. doi: 10.1038/nchembio.2312. Epub 2017 Feb 13.

Backbone

• Vector backbone: pEvoltac
• Backbone size w/o insert (bp): 4166
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ATMW1
• Growth instructions: This plasmid can be grown in either ATMW1 or ATMW-BL21 strains (Addgene #218768 or 218769)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EcWRS-h9
• Species: E. coli
• Insert Size (bp): 1005
• Mutation: S8A, V144S, V146A
• Promoter: tacI

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pevoltac-ecw-tga-h9.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EcW-tRNA-TGA

After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.