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Addgene

pEvoltac-EcW-TGA-h14
(Plasmid #218764)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218764 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEvoltac
  • Backbone size w/o insert (bp) 4166
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ATMW1
  • Growth instructions
    This plasmid can be grown in either ATMW1 or ATMW-BL21 strains (Addgene #218768 or 218769)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EcWRS-h14
  • Species
    E. coli
  • Insert Size (bp)
    1005
  • Mutation
    S8A, V144G, V146C
  • Promoter tacI

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EcW-tRNA-TGA

After the plasmids are propagated, they need to be gel purified by agarose gel because the ATM strains contain a plasmid in their genome.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEvoltac-EcW-TGA-h14 was a gift from Abhishek Chatterjee (Addgene plasmid # 218764 ; http://n2t.net/addgene:218764 ; RRID:Addgene_218764)

  • For your References section:

    An orthogonalized platform for genetic code expansion in both bacteria and eukaryotes. Italia JS, Addy PS, Wrobel CJ, Crawford LA, Lajoie MJ, Zheng Y, Chatterjee A. Nat Chem Biol. 2017 Apr;13(4):446-450. doi: 10.1038/nchembio.2312. Epub 2017 Feb 13. 10.1038/nchembio.2312 PubMed 28192410
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