pAAV-hSyn-ConVERGD-N2cG-WPRE
(Plasmid
#218757)
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PurposeProvides AND intersectional (Cre+Flp) expression of N2C glycoprotein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218757 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerAzenta Life Sciences
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameN2cG
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SpeciesRabies virus
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MutationSee Depositor Comments
- Promoter hSyn
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.02.07.527312 for bioRxiv preprint.
Addgene's NGS results found a H358P mutation in the N2cG insert that does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-ConVERGD-N2cG-WPRE was a gift from Lindsay Schwarz (Addgene plasmid # 218757 ; http://n2t.net/addgene:218757 ; RRID:Addgene_218757) -
For your References section:
A Novel Single Vector Intersectional AAV Strategy for Interrogating Cellular Diversity and Brain Function. Hughes AC, Pollard BG, Xu B, Gammons JW, Chapman P, Bikoff JB, Schwarz LA. bioRxiv. 2023 Feb 8:2023.02.07.527312. doi: 10.1101/2023.02.07.527312. Preprint. 10.1101/2023.02.07.527312 PubMed 36798174