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pCAG2-EGFP-C-IFT122
(Plasmid #218737)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218737 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG2-EGFP-C
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5946
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IFT122
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3546
  • Entrez Gene
    IFT122 (a.k.a. CED, CED1, CFAP80, FAP80, SPG, WDR10, WDR10p, WDR140)
  • Promoter CAG
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CTGAGCAAAGACCCCAACGAG
  • 3′ sequencing primer GCCAGAAGTCAGATGCTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid expresses transcript variant 3, which lacks two alternate in-frame exons in the 5' coding region, compared to variant 1. It encodes isoform 3. RefSeq : NM_018262.4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG2-EGFP-C-IFT122 was a gift from Kazuhisa Nakayama (Addgene plasmid # 218737 ; http://n2t.net/addgene:218737 ; RRID:Addgene_218737)
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