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pCAG-EGFP-C-IFT70B
(Plasmid #218729)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218729 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-EGFP-C
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6767
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IFT70B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1878
  • Entrez Gene
    TTC30B (a.k.a. IFT70, IFT70B, fleer)
  • Promoter CAG
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII/BamHI (destroyed during cloning)
  • 3′ cloning site XhoI/SalI (destroyed during cloning)
  • 5′ sequencing primer CTGAGCAAAGACCCCAACGAG
  • 3′ sequencing primer GCCAGAAGTCAGATGCTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-EGFP-C-IFT70B was a gift from Kazuhisa Nakayama (Addgene plasmid # 218729 ; http://n2t.net/addgene:218729 ; RRID:Addgene_218729)

  • For your References section:

    Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis. Takei R, Katoh Y, Nakayama K. Biol Open. 2018 Apr 30;7(5). pii: bio.033241. doi: 10.1242/bio.033241. 10.1242/bio.033241 PubMed 29654116
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