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Addgene

pEGFP-C1-BBS4
(Plasmid #218713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BBS4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1557
  • Mutation
    I354T (t1110c)
  • Entrez Gene
    BBS4
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII/BamHI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CTGAGCAAAGACCCCAACGAG
  • 3′ sequencing primer GAAATTTGTGATGCTATTGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-BBS4 was a gift from Kazuhisa Nakayama (Addgene plasmid # 218713 ; http://n2t.net/addgene:218713 ; RRID:Addgene_218713)

  • For your References section:

    Multisubunit complex architectures revealed by visible immunoprecipitation (VIP) assay using fluorescent fusion proteins. Katoh Y, Nozaki S, Hartanto D, Miyano R, Nakayama K. J Cell Sci. 2015 May 11. pii: jcs.168740. 10.1242/jcs.168740 PubMed 25964651
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