pCDB179-PES-H1-L92F-Q94Y
(Plasmid
#218705)
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PurposeEncodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDB179
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePES-H1
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SpeciesSynthetic
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MutationL92F-Q94Y
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Tag
/ Fusion Protein
- 10xHis (N terminal on backbone)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDB179-PES-H1-L92F-Q94Y was a gift from Gregg Beckham (Addgene plasmid # 218705 ; http://n2t.net/addgene:218705 ; RRID:Addgene_218705)