AAV-SYN-mBaoJin-oPRE
(Plasmid
#218683)
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PurposeAAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218683 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneN/A
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Backbone manufacturerVectorBuilder
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemBaoJin
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Alt namemBJ
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Alt nameMonomer variant of StayGold
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SpeciesC. uchidae
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Insert Size (bp)705
- Promoter Human synapsin promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVectorBuilder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The DNA sequence for mBaoJin is based on supplementary figure 2 in DOI: 10.1038/s41592-024-02203-y.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-SYN-mBaoJin-oPRE was a gift from Bart Nieuwenhuis (Addgene plasmid # 218683 ; http://n2t.net/addgene:218683 ; RRID:Addgene_218683)
