vGlut1 peptide- GST
(Plasmid
#218677)
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PurposeExpresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesicles
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218677 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepD454
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Backbone manufacturerDNA 2.0
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namevGlut
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SpeciesR. norvegicus (rat)
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Entrez GeneSlc17a6 (a.k.a. 2900073D12Rik, DNPI, VGLUT2)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
vGlut1 peptide- GST was a gift from Axel Brunger (Addgene plasmid # 218677 ; http://n2t.net/addgene:218677 ; RRID:Addgene_218677) -
For your References section:
Observing isolated synaptic vesicle association and fusion ex vivo. Leitz J, Wang C, Esquivies L, Peters JJ, Gopal N, Pfuetzner RA, Wang AL, Brunger AT. Nat Protoc. 2024 Jul 2. doi: 10.1038/s41596-024-01014-x. 10.1038/s41596-024-01014-x PubMed 38956381