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Addgene

C1C2CMUNC2C
(Plasmid #218674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MUNC13
  • Species
    R. norvegicus (rat)
  • Mutation
    Amino acids1407-1453 deleted
  • GenBank ID
    64829
  • Entrez Gene
    Unc13a (a.k.a. Munc13-1, Unc13h1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C1C2CMUNC2C was a gift from Axel Brunger (Addgene plasmid # 218674 ; http://n2t.net/addgene:218674 ; RRID:Addgene_218674)

  • For your References section:

    Observing isolated synaptic vesicle association and fusion ex vivo. Leitz J, Wang C, Esquivies L, Peters JJ, Gopal N, Pfuetzner RA, Wang AL, Brunger AT. Nat Protoc. 2024 Jul 2. doi: 10.1038/s41596-024-01014-x. 10.1038/s41596-024-01014-x PubMed 38956381
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