C1C2CMUNC2C
(Plasmid
#218674)
-
PurposeInsect cell expression of a rat MUNC13-1 fragment containing domains C1C2BMUNC2C
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 218674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFastBac
-
Vector typeInsect Expression
-
Selectable markersGentamicin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameMUNC13
-
SpeciesR. norvegicus (rat)
-
MutationAmino acids1407-1453 deleted
-
GenBank ID64829
-
Entrez GeneUnc13a (a.k.a. Munc13-1, Unc13h1)
-
Tag
/ Fusion Protein
- GST (N terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
C1C2CMUNC2C was a gift from Axel Brunger (Addgene plasmid # 218674 ; http://n2t.net/addgene:218674 ; RRID:Addgene_218674) -
For your References section:
Observing isolated synaptic vesicle association and fusion ex vivo. Leitz J, Wang C, Esquivies L, Peters JJ, Gopal N, Pfuetzner RA, Wang AL, Brunger AT. Nat Protoc. 2024 Jul 2. doi: 10.1038/s41596-024-01014-x. 10.1038/s41596-024-01014-x PubMed 38956381