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Addgene

pDC180
(Plasmid #218673)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218673 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJExpress414
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rat ⍺SNAP
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Napa (a.k.a. alpha-SNAP)
  • Tag / Fusion Protein
    • 6 his (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDC180 was a gift from Axel Brunger (Addgene plasmid # 218673 ; http://n2t.net/addgene:218673 ; RRID:Addgene_218673)

  • For your References section:

    Observing isolated synaptic vesicle association and fusion ex vivo. Leitz J, Wang C, Esquivies L, Peters JJ, Gopal N, Pfuetzner RA, Wang AL, Brunger AT. Nat Protoc. 2024 Jul 2. doi: 10.1038/s41596-024-01014-x. 10.1038/s41596-024-01014-x PubMed 38956381
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