pBAD33-mf-lon
(Plasmid #21867)

• Depositing Lab: Robert Sauer
• Publication: Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 105(42):16113-8.

Backbone

• Vector backbone: pBAD33
• Backbone manufacturer: Beckwith Lab
• Backbone size w/o insert (bp): 5352
• Modifications to backbone: The origin is pBR322 not p15a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lon
• Species: Mesoplasma florum
• Insert Size (bp): 2326
• Mutation: all TGA codons changed to TGG codons
• Entrez Gene: Mfl404 (a.k.a. Mfl404)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pBAD-fwd
• 3′ sequencing primer: pBAD-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A detailed protocol for purification of mf-lon can be found in the associated paper.