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Addgene

plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA
(Plasmid #218666)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218666 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    plentiCRISPR-v2 Puro
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EIF2AK3
  • Alt name
    PERK
  • gRNA/shRNA sequence
    5’-GCAGAGGCCGGGCTGAGACG-3’
  • Species
    H. sapiens (human)
  • Entrez Gene
    EIF2AK3 (a.k.a. PEK, PERK, WRS)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA was a gift from Kivanc Birsoy (Addgene plasmid # 218666 ; http://n2t.net/addgene:218666 ; RRID:Addgene_218666)

  • For your References section:

    Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals. Kenny TC, Khan A, Son Y, Yue L, Heissel S, Sharma A, Pasolli HA, Liu Y, Gamazon ER, Alwaseem H, Hite RK, Birsoy K. Cell Metab. 2023 Jun 6;35(6):1057-1071.e12. doi: 10.1016/j.cmet.2023.04.003. Epub 2023 Apr 25. 10.1016/j.cmet.2023.04.003 PubMed 37100056
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