Addgene: ATF4 19: (SL)RLuc.5’ATF4.GFP Skip to main content
Addgene

ATF4 19: (SL)RLuc.5’ATF4.GFP
(Plasmid #21865)

Full plasmid sequence is not available for this item.

Created with RaphaëlATF4 19: (SL)RLuc.5’ATF4.GFPEGFPATF4

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21865 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATF4
  • Species
    M. musculus (mouse)
  • Mutation
    Stem Loop upstream of ATF4
  • Entrez Gene
    Atf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATF4 19: (SL)RLuc.5’ATF4.GFP was a gift from David Ron (Addgene plasmid # 21865 ; http://n2t.net/addgene:21865 ; RRID:Addgene_21865)
  • For your References section:

    Translation reinitiation at alternative open reading frames regulates gene expression in an integrated stress response. Lu PD, Harding HP, Ron D. J Cell Biol. 2004 Oct 11. 167(1):27-33. 10.1083/jcb.200408003 PubMed 15479734