ATF4 18: RLuc.5’ATF4.GFP
(Plasmid
#21864)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl2
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Growth instructionsStbl2
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATF4
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SpeciesM. musculus (mouse)
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Entrez GeneAtf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ATF4 18: RLuc.5’ATF4.GFP was a gift from David Ron (Addgene plasmid # 21864 ; http://n2t.net/addgene:21864 ; RRID:Addgene_21864) -
For your References section:
Translation reinitiation at alternative open reading frames regulates gene expression in an integrated stress response. Lu PD, Harding HP, Ron D. J Cell Biol. 2004 Oct 11. 167(1):27-33. 10.1083/jcb.200408003 PubMed 15479734