pLVX-TetOne-Puro-mitoEcSTH-FLAG
(Plasmid #218629)

• Purpose: This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
• Depositing Lab: Valentin Cracan
• Publication: Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.

Backbone

• Vector backbone: pLVX-TetOne-Puro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 9227
• Total vector size (bp): 10763
• Vector type: Mammalian Expression, Lentiviral, Synthetic Biology
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mitoEcSTH
• Species: E. coli
• Insert Size (bp): 1536
• Tags / Fusion Proteins:
  • FLAG (C terminal on insert)
  • MTS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: ACGGCCCTCCTGTCTTAGGTTAGTGAAAAATGT
• 3′ sequencing primer: CTTTGCTTATGTAAACCAGGGCGCCTAT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry