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Addgene

pJJB1561
(Plasmid #218626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218626 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TMP138
  • Backbone manufacturer
    Internal
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GES
  • Alt name
    Geraniol synthase
  • Species
    S. cerevisiae (budding yeast); Valeriana officinalis
  • Tag / Fusion Protein
    • ePTS1 (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Express geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags to guide the proteins to the peroxisome and cytosolic Erg20_WT_synthetic homodimer. Integrated into URA3 locus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJB1561 was a gift from John Dueber (Addgene plasmid # 218626 ; http://n2t.net/addgene:218626 ; RRID:Addgene_218626)
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