pJJB1465
(Plasmid
#218625)
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PurposeExpress engineered TFs Adr1c(S230A), Pip2c, and Oaf1c
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218625 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTMP136
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Backbone manufacturerInternal
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAdr1
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses three engineered transcription factors (TFs): Adr1c, Pip2c, and Oaf1c for inducing peroxisome proliferation consitutively. Integrated into the HIS3 locus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJJB1465 was a gift from John Dueber (Addgene plasmid # 218625 ; http://n2t.net/addgene:218625 ; RRID:Addgene_218625)