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Addgene

pJJB1462
(Plasmid #218623)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218623 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TMP136
  • Backbone manufacturer
    Internal
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3 ; mKate, YFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP
  • Species
    S. cerevisiae (budding yeast)
  • Tag / Fusion Protein
    • ePTS1 (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease. Used for competition assay for import into peroxisome. Integrated into HIS3 locus using HIS3 selection marker.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJB1462 was a gift from John Dueber (Addgene plasmid # 218623 ; http://n2t.net/addgene:218623 ; RRID:Addgene_218623)
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