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Addgene

pJJB1426
(Plasmid #218622)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218622 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PSG301
  • Backbone manufacturer
    Internal
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3 ; YFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tNCS
  • Species
    Camellia japonica
  • Mutation
    106-588
  • Tag / Fusion Protein
    • YFP-ePTS1 (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used for toxicity assay. Express the toxic, truncated tNCS enzyme with a YFP and ePTS1 tag to direct the fusion to the peroxisome. This is done on a high copy number 2 micron plasmid using HIS3 as the selectable marker.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJB1426 was a gift from John Dueber (Addgene plasmid # 218622 ; http://n2t.net/addgene:218622 ; RRID:Addgene_218622)
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