pJJB1536
(Plasmid
#218617)
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PurposeExpress Pex5 and Pex11
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 218617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePSG747
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Backbone manufacturerInternal
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Vector typeYeast Expression
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Selectable markersMarkerless integration into Saccharomyces cerevisiae YBR197C locus using Cas9
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePex5
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Integrate Pex5 and Pex11 into the YBR197C locus
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJJB1536 was a gift from John Dueber (Addgene plasmid # 218617 ; http://n2t.net/addgene:218617 ; RRID:Addgene_218617)