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Addgene

pSKT001
(Plasmid #218594)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218594 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PSG746
  • Backbone manufacturer
    Internal
  • Vector type
    Yeast Expression
  • Selectable markers
    YFP. Markerless integration into Saccharomyces cerevisiae IAI11 locus using Cas9

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP
  • Species
    S. cerevisiae (budding yeast)
  • Tags / Fusion Proteins
    • UbiY degradation tag (N terminal on insert)
    • ePTS1 (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses the components for the degron assay: UbiY(N-terminal degradation tag)-YFP-ePTS1, directing the YFP to the peroxisome. Integrated into the IAI11 locus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSKT001 was a gift from John Dueber (Addgene plasmid # 218594 ; http://n2t.net/addgene:218594 ; RRID:Addgene_218594)
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