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Addgene

pJJB1340
(Plasmid #218593)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218593 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TMP137
  • Backbone manufacturer
    Internal
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2 ; mRuby2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pex22
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Pex22(1-36)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses Pex22(1-36)-mRuby2 which directs mRuby2 to the peroxisome membrane. Also overexpresses Pex11p. Integrated into the LEU2 locus

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJB1340 was a gift from John Dueber (Addgene plasmid # 218593 ; http://n2t.net/addgene:218593 ; RRID:Addgene_218593)
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