pJJB1338
(Plasmid
#218592)
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PurposeExpress a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218592 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTMP137
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Backbone manufacturerInternal
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Vector typeYeast Expression
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Selectable markersLEU2 ; mRuby2
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePex22
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SpeciesS. cerevisiae (budding yeast)
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MutationPex22(1-36)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses Pex22(1-36)-mRuby2 which directs mRuby2 to the peroxisome membrane. Integrated into the LEU2 locus
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJJB1338 was a gift from John Dueber (Addgene plasmid # 218592 ; http://n2t.net/addgene:218592 ; RRID:Addgene_218592)