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Addgene

pJJB170
(Plasmid #218591)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218591 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TMP137
  • Backbone manufacturer
    Internal
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Adr1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Adr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-300 + 2,419-3,141)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses three engineered transcription factors (TFs): Adr1c, Pip2c, and Oaf1c for inducing peroxisome proliferation consitutively. Integrated into the LEU2 locus

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJB170 was a gift from John Dueber (Addgene plasmid # 218591 ; http://n2t.net/addgene:218591 ; RRID:Addgene_218591)
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