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Addgene

pJJB1439
(Plasmid #218589)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218589 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TMP138
  • Backbone manufacturer
    Internal
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GES
  • Alt name
    Geraniol synthase
  • Species
    S. cerevisiae (budding yeast); Valeriana officinalis
  • Mutation
    Erg20(F96W, N127W)
  • Tag / Fusion Protein
    • ePTS1 (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also expresses Idi1 and a mutant Erg20 (F96W, N127W) from Saccharomyces cerevisiae. Integrated into the URA3 locus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJB1439 was a gift from John Dueber (Addgene plasmid # 218589 ; http://n2t.net/addgene:218589 ; RRID:Addgene_218589)
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