pKS1469
(Plasmid
#218588)
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PurposeExpress peroxisomal MvaSp and MvaEp to convert AcCoA to mevalonate
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTMP137
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Backbone manufacturerInternal
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMvaS
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SpeciesEnterococcus faecalis
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Tag
/ Fusion Protein
- ePTS1 (C terminal on insert)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also expresses MvaE from Enteroccocus faecalis. Integrated into the LEU2 locus
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKS1469 was a gift from John Dueber (Addgene plasmid # 218588 ; http://n2t.net/addgene:218588 ; RRID:Addgene_218588)