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Addgene

pKS1461
(Plasmid #218587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PSG749
  • Backbone manufacturer
    Internal
  • Vector type
    Yeast Expression
  • Selectable markers
    Markerless integration into Saccharomyces cerevisiae YDR514C locus using Cas9

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Erg12
  • Species
    S. cerevisiae (budding yeast)
  • Tag / Fusion Protein
    • ePTS1 (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also expresses Erg8p and Mvd1p from Saccharomyces cerevisiae. Integrated into the YDR514C locus

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKS1461 was a gift from John Dueber (Addgene plasmid # 218587 ; http://n2t.net/addgene:218587 ; RRID:Addgene_218587)
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