peGFP C3-SPG20-PAAA
(Plasmid #218581)

• Purpose: Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH
• Depositing Lab: Hemmo Meyer
• Publication: Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)00172-2. doi: 10.1016/j.molcel.2024.02.029.

Backbone

• Vector backbone: peGFP-C3
• Total vector size (bp): 6695
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SPG20
• Alt name: Spartin
• Species: H. sapiens (human)
• Insert Size (bp): 2001
• Mutation: changed Proline 172 to Alanine and Tyrosine 174 to Alanine
• Entrez Gene: SPART (a.k.a. SPG20, TAHCCP1)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: CMVfor

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry