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peGFP C3-SPG20 8xV
(Plasmid #218579)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218579 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peGFP-C3
  • Total vector size (bp) 6696
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SPG20
  • Alt name
    Spartin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2001
  • Mutation
    changed Tryptophan 419, Leucine 438, Isoleucine 449, Leucine 456, Phenylalanine 549, Leucine 556, Isoleucine 563 and Isoleucine 609 to Valine
  • Entrez Gene
    SPART (a.k.a. SPG20, TAHCCP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    peGFP C3-SPG20 8xV was a gift from Hemmo Meyer (Addgene plasmid # 218579 ; http://n2t.net/addgene:218579 ; RRID:Addgene_218579)

  • For your References section:

    Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH. Gahlot P, Kravic B, Rota G, van den Boom J, Levantovsky S, Schulze N, Maspero E, Polo S, Behrends C, Meyer H. Mol Cell. 2024 Mar 14:S1097-2765(24)00172-2. doi: 10.1016/j.molcel.2024.02.029. 10.1016/j.molcel.2024.02.029 PubMed 38503285
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