peGFP C3-SPG20
(Plasmid
#218578)
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PurposeExpresses GFP-tagged human SPG20 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218578 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepeGFP-C3
- Total vector size (bp) 6696
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSPG20
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Alt nameSpartin
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SpeciesH. sapiens (human)
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Insert Size (bp)2001
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Entrez GeneSPART (a.k.a. SPG20, TAHCCP1)
- Promoter CMV
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HINDIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMVfor (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
peGFP C3-SPG20 was a gift from Hemmo Meyer (Addgene plasmid # 218578 ; http://n2t.net/addgene:218578 ; RRID:Addgene_218578) -
For your References section:
Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH. Gahlot P, Kravic B, Rota G, van den Boom J, Levantovsky S, Schulze N, Maspero E, Polo S, Behrends C, Meyer H. Mol Cell. 2024 Mar 14:S1097-2765(24)00172-2. doi: 10.1016/j.molcel.2024.02.029. 10.1016/j.molcel.2024.02.029 PubMed 38503285
