plentiCRISPR-v2 Puro FLVCR1_sg5
(Plasmid
#218522)
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PurposesgRNA targeting human FLVCR1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneplentiCRISPR-v2 Puro
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Vector typeLentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLVCR1
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Alt nameMFSD7b
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gRNA/shRNA sequence5’-GTAGGCCAGCATGTACACCA-3’
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SpeciesH. sapiens (human)
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Entrez GeneFLVCR1 (a.k.a. AXPC1, FLVCR, MFSD7B, PCA, PCARP, SLC49A1)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plentiCRISPR-v2 Puro FLVCR1_sg5 was a gift from Kivanc Birsoy (Addgene plasmid # 218522 ; http://n2t.net/addgene:218522 ; RRID:Addgene_218522) -
For your References section:
Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals. Kenny TC, Khan A, Son Y, Yue L, Heissel S, Sharma A, Pasolli HA, Liu Y, Gamazon ER, Alwaseem H, Hite RK, Birsoy K. Cell Metab. 2023 Jun 6;35(6):1057-1071.e12. doi: 10.1016/j.cmet.2023.04.003. Epub 2023 Apr 25. 10.1016/j.cmet.2023.04.003 PubMed 37100056