ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc
(Plasmid #21850)

• Depositing Lab: David Ron
• Publication: Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

Backbone

• Vector backbone: pGL3
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5010
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ATF4
• Species: M. musculus (mouse)
• Insert Size (bp): 101
• Mutation: ATF4 5' UTR and AUG fused to Luciferase driven by the TK promoter
• Entrez Gene: Atf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: RVprimer3

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results show 2 mismatches with author's sequence and NCBI sequence for ATF4.

Mouse ATF4 5' UTR and AUG fused to Luciferase driven by the TK promoter in a pGL3 backbone

TK.mATF4.UTR.Luc.pGL3 (5202nt, predicted). TK promoter: 58..184; mATF4 5'UTR and AUG: 185..470; luciferase coding sequence: 472..2121; poly A signals: 2156..2377