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ATF4 2: Mouse ATF4 (CHOP11/cATF)-bZIP
(Plasmid #21849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21849 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATF4
  • Species
    M. musculus (mouse)
  • Mutation
    N-terminal transactivation domain deleted and replaced with a 9E10 (Myc) epitope tag
  • Entrez Gene
    Atf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse ATF4, bZIP domain only; N-terminal transactivation domain deleted and replaced with a 9E10 (Myc) epitope tag, mammalian expression vector pCDNA3, tagged with hCD2 surface marker that replaces the Neo gene (see: Harding, H., et. al Mol Cell 6, November 2000 issue for details)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATF4 2: Mouse ATF4 (CHOP11/cATF)-bZIP was a gift from David Ron (Addgene plasmid # 21849 ; http://n2t.net/addgene:21849 ; RRID:Addgene_21849)
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