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Addgene

JO-pH720-SARS-CoV-2-S-tail-Cys1254Ala-Thr1273Glu
(Plasmid #218454)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218454 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pH720
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag and Cys1254Ala and Thr1273Glu substitutions
  • Alt name
    Cientized Spike Tail-NMR
  • Species
    SARS-CoV-2
  • GenBank ID
    43740568
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tag / Fusion Protein
    • eXact (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JO-pH720-SARS-CoV-2-S-tail-Cys1254Ala-Thr1273Glu was a gift from Syed Saif Hasan (Addgene plasmid # 218454 ; http://n2t.net/addgene:218454 ; RRID:Addgene_218454)

  • For your References section:

    A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs. Dey D, Qing E, He Y, Chen Y, Jennings B, Cohn W, Singh S, Gakhar L, Schnicker NJ, Pierce BG, Whitelegge JP, Doray B, Orban J, Gallagher T, Hasan SS. Nat Commun. 2023 Dec 15;14(1):8358. doi: 10.1038/s41467-023-44076-3. 10.1038/s41467-023-44076-3 PubMed 38102143
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