ATF4 1: Mouse ATF4 (CHOP11/cATF)-WT
(Plasmid #21845)

• Depositing Lab: David Ron
• Publication: ATF4 plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATF4
• Species: M. musculus (mouse)
• Insert Size (bp): 1609
• Entrez Gene: Atf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • BglII & KpnI digests
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mouse ATF4, wild-type, deleted of translational leader, insert assembled from clone CHOP11/cATF (Vallejo, M., et al. Proc Natl Acad Sci U S A 90, 4679-83), mammalian expression vector pCDNA3, tagged with hCD2 surface marker that replaces the Neo gene (see: Harding, H., et. al Mol Cell 6, November 2000 issue for details)