Skip to main content
Addgene

pcDNA5_NLS-Halo-eGFP-BioID
(Plasmid #218448)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218448 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA5
  • Backbone size w/o insert (bp) 5084
  • Total vector size (bp) 7709
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NLS-Halo-eGFP-BirA
  • Species
    E. coli, Rhodococcus rhodochrous, Aequorea victoria
  • Insert Size (bp)
    2625
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer TTAAGCTTGGTACCGAGCTCGGATCC
  • 3′ sequencing primer GATGGCTGGCAACTAGAAGGCACAG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5_NLS-Halo-eGFP-BioID was a gift from Thorsten Stafforst (Addgene plasmid # 218448 ; http://n2t.net/addgene:218448 ; RRID:Addgene_218448)

  • For your References section:

    Profiling the interactome of oligonucleotide drugs by proximity biotinylation. Hanswillemenke A, Hofacker DT, Sorgenfrei M, Fruhner C, Franz-Wachtel M, Schwarzer D, Macek B, Stafforst T. Nat Chem Biol. 2024 Jan 17. doi: 10.1038/s41589-023-01530-z. 10.1038/s41589-023-01530-z PubMed 38233583
Related items: