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gWiz_BM40-vGAT-VL-hIgG1-CL
(Plasmid #218437)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218437 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    gWiz
  • Vector type
    Mammalian Expression, Affinity Reagent/ Antibody

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    vGAT-VL-hIgG1-CL
  • Alt name
    light chain fragment of an anti-vGAT Fab domain
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • BM40 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    NeuroMab Seq (https://neuromabseq.ucdavis.edu/)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequence derived from NeruoMab Clone #: L118/80 - NeuroMab Seq is supported by grant NIH U24 NS109113.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gWiz_BM40-vGAT-VL-hIgG1-CL was a gift from Axel Brunger (Addgene plasmid # 218437 ; http://n2t.net/addgene:218437 ; RRID:Addgene_218437)

  • For your References section:

    A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles. Gopal N, Leitz J, Wang C, Esquivies L, Pfuetzner RA, Brunger AT. Curr Res Physiol. 2024 Feb 23;7:100121. doi: 10.1016/j.crphys.2024.100121. eCollection 2024. 10.1016/j.crphys.2024.100121 PubMed 38572021
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