MUT_S1-N
(Plasmid
#218398)
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Purpose3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUHD10-3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameModified CHO DHFR minigene
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SpeciesSynthetic
- Promoter tetracycline-responsive promoter
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer LNCX
- 3′ sequencing primer EBV Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MUT_S1-N was a gift from Oded Regev (Addgene plasmid # 218398 ; http://n2t.net/addgene:218398 ; RRID:Addgene_218398)
