Skip to main content
Addgene

AMT1KO
(Plasmid #218373)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218373 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    psk minus
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 8700
  • Vector type
    Unspecified
  • Selectable markers
    Paromomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AMT1
  • Species
    Tetrahymena thermophila
  • Insert Size (bp)
    2052
  • Mutation
    deleted complete AMT1 gene
  • GenBank ID
    NW_002476174

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer GAGCTC CATCTGATTGATGGCTTCTCTATC
  • 3′ sequencing primer GGTACC CCTCTGCCTTAGCACTTTAGCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AMT1KO was a gift from Shan Gao (Addgene plasmid # 218373 ; http://n2t.net/addgene:218373 ; RRID:Addgene_218373)
Related items: